MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue

MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue

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Summary: Transcription factor MAFB regulates various homeostatic functions of macrophages.This study explores the SEIMENS WI14W500GB INTEGRATED WASHING MACHINE role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f::LysM-Cre) mice.We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions.

This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT.We confirm MAFB regulation of IL-6 expression using luciferase readout driven by IL-6 promoter in RAW-264.7 macrophage cell lines.

Immunohistochemistry shows clustered organization of NGF-producing cells in BAT, which are primarily TRPV1+ vascular smooth muscle cells, as additionally Hose Sink Hook shown using single-cell RNA sequencing and RT-qPCR of the stromal vascular fraction.Treating Mafbf/f::LysM-Cre mice with anti-IL-6 receptor antibody rescues SN density, body temperature, and energy expenditure.